Prodigiosin production by Serratia marcescens UCP 1549 using renewable-resources as a low cost substrate.

نویسندگان

  • Helvia W Casullo de Araújo
  • K Fukushima
  • Galba M Campos Takaki
چکیده

A new strain of Serratia marcescens UCP1459 isolated from a semi-arid soil produced the natural red pigment prodigiosin, characterized by an uncommon pyrrolylpyrromethane skeleton. Prodigiosin is a promising drug due to its reported antifungal, immunosuppressive and anti-proliferative activities. The objective of this work was to indentify a suitable medium to simultaneously enhance S. marcescens growth and pigment production using renewable resources obtained from industrial wastes. S. marcescens produced the highest level of prodigiosin (49.5 g/L) at 48 h of cultivation using 6% "manipueira" (cassava wastewater) supplemented with mannitol (2%) at pH 7 and 28 °C. Carbohydrates in "manipueira" and mannitol play a role in the enhanced cell growth and prodigiosin production. The purified pigment extracted from the biomass was analyzed by mass spectrophotometry and showed the expected molecular weight of 324 Da corresponding to prodigiosin. In conclusion, we have successfully designed a new, economically feasible medium supporting enhanced S. marcescens growth and a high yield production of prodigiosin.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone

This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper (Poecilemon tauricola Ramme 1951), which was collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA classified the microbe as Serratia...

متن کامل

Isolation of Pigment-Producing Bacteria and Characterization of the Extracted Pigments

Bacteria produce pigments for various reasons and it plays an important role. Some bacteria such as cyanobacteria have phycobilin pigments to carry out photosynthesis. Other example for pigment-producing bacterial strains includes Serratia marcescens that produces prodigiosin, Streptomyces coelicolor (prodigiosin and actinorhodin), Chromobacterium violaceum (violacein) and Thialkalivibrio versu...

متن کامل

Isolation of Pigment-Producing Bacteria and Characterization of the Extracted Pigments

Bacteria produce pigments for various reasons and it plays an important role. Some bacteria such as cyanobacteria have phycobilin pigments to carry out photosynthesis. Other example for pigment-producing bacterial strains includes Serratia marcescens that produces prodigiosin, Streptomyces coelicolor (prodigiosin and actinorhodin), Chromobacterium violaceum (violacein) and Thialkalivibrio versu...

متن کامل

Isolation of Pigment-Producing Bacteria and Characterization of the Extracted Pigments

Bacteria produce pigments for various reasons and it plays an important role. Some bacteria such as cyanobacteria have phycobilin pigments to carry out photosynthesis. Other example for pigment-producing bacterial strains includes Serratia marcescens that produces prodigiosin, Streptomyces coelicolor (prodigiosin and actinorhodin), Chromobacterium violaceum (violacein) and Thialkalivibrio versu...

متن کامل

Oxidation of dibenzothiophene (DBT) by Serratia marcescens UCP 1549 formed biphenyl as final product

UNLABELLED BACKGROUND The desulphurization of dibenzothiophene (DBT), a recalcitrant thiophenic fossil fuel component by Serratia marcescens (UCP 1549) in order for reducing the Sulphur content was investigated. The Study was carried out establishing the growth profile using Luria Bertani medium to different concentrations of DBT during 120 hours at 28°C, and orbital Shaker at 150 rpm. RES...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Molecules

دوره 15 10  شماره 

صفحات  -

تاریخ انتشار 2010